Streptavidin Protein (PE)
Quick Overview for Streptavidin Protein (PE) (ABIN964539)
抗原
See all Streptavidin 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This Streptavidin protein is labelled with PE.
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Supplier Product No.
- s000-08
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Supplier
- Rockland
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原理
- Streptavidin Phycoerythrin Conjugated
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纯化方法
- Streptavidin-Phycoerythrin was prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin. Free fluorochrome is removed by tandem molecular sieve chromatography.
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过滤
- Sterile filtered
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Streptavidin protein (HRP)
宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, ELISA, FM, IHC, DB, IF, IEM
Streptavidin (AA 25-183) protein (His tag)宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Streptavidin (AA 37-159) protein (His tag)宿主: Streptomyces 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Streptavidin protein (TRITC)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native FM, FLISA, IHC, FISH
Streptavidin protein (Atto 425)宿主: Streptomyces avidinii 宿主: Streptomyces avidinii Native WB, FM, FLISA, DB, IF
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应用备注
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Flow Cytometry Dilution: 1:100 - 1:250
Application Note: Phycoerythrin conjugated streptavidin has been tested by dot blot and FACS and is suitable for immunomicroscopy, flow cytometry or FACS analysis, as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c)
IF Microscopy Dilution: 1:100 - 1:250
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 1.0 mL)
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浓度
- 0.5 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room tempe Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Streptavidin
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背景
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Synonyms: Streptavidin PE, Phyco Streptavidin, SA Phycoerythrin, R-Phycoerythrin, Phycoerythrin conjugated streptavidin
Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditio
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UniProt
- P22629
抗原
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